Intact Genomics (IG) i7 High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace (1). It is ideal for cloning and can be used for long (up to 20kb) or difficult amplicons. This product is supplied with the Intact Genomics proprietary 2.5x i7 PCR reaction buffer containing MgCl2 with a final (1x) concentration of 2 mM. This buffer allows for amplification of non GC rich templates and of GC rich templates up to 84%.Applications Long and difficult template DNA amplification Cloning High-fidelity PCR Efficient for amplifying high GC content template DNA with magic enhancer